Tryptase

Ordering Recommendation

Measure total tryptase to confirm mast cell activation in diseases such as mastocytosis, anaphylaxis, urticaria, and asthma. Not generally used acutely except where the diagnosis is unclear. Useful in the prognosis of systemic mastocytosis.

Mnemonic

EGGWHOLE

Methodology

Quantitative ImmunoCAP Fluorescent Enzyme Immunoassay

Performed

Mon-Sat

Reported

1-2 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation:

Collect:

Serum separator tube (SST).

Specimen Preparation:

Allow serum to clot completely at room temperature. Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to a Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature:

Frozen.

Unacceptable Conditions:

Hemolyzed, icteric, or lipemic specimens.

Remarks:

Stability:

After separation from cells: Ambient: 48 hours; Refrigerated: 72 hours; Frozen: 1 month

Reference Interval

Less than 11.0 µg/L

Interpretive Data

Compliance Category: FDA

Note

This test measures total tryptase and does not distinguish between the alpha and beta protein types. Samples should preferably be collected between 15 minutes and three hours after the event suspected to have caused mast cell activation.

Tryptase

Ordering Recommendation

Mnemonic

EGGWHOLE

Methodology

Quantitative ImmunoCAP Fluorescent Enzyme Immunoassay

Performed

Mon-Sat

Reported

1-2 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation:

Collect:

Serum separator tube (SST).

Specimen Preparation:

Allow serum to clot completely at room temperature. Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to a Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature:

Frozen.

Unacceptable Conditions:

Hemolyzed, icteric, or lipemic specimens.

Remarks:

Stability:

After separation from cells: Ambient: 48 hours; Refrigerated: 72 hours; Frozen: 1 month

Reference Interval

Less than 11.0 µg/L

Interpretive Data

Compliance Category: FDA

Note

This test measures total tryptase and does not distinguish between the alpha and beta protein types. Samples should preferably be collected between 15 minutes and three hours after the event suspected to have caused mast cell activation.

CPT Code

83520

Components

Component Chart Name

Tryptase

Component Test Code

0099173

LOINC

21582-2

Aliases

Total Tryptase Tryptase, Autopsy (Tryptase)

Total Tryptase
Tryptase, Autopsy (Tryptase)