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Ordering information regarding a Erythrocyte Sedimentation Rate test for Clinical Diagnostic Laboratories. Erythrocyte Sedimentation Rate (ESR) Ordering Recommendation Methodology The ESR test measures the rate at which red blood cells (erythrocytes) settle at the bottom of a test tube over a period of one hour. Inflammation causes red blood cells to clump together, making them heavier and causing them to fall more quickly1. The distance the red blood cells fall is measured and reported as the ESR. Performed Mon-Sat Reported Within 24 hours New York DOH Approval Status This test is New York DOH approved. Specimen Required Patient Preparation: Collect: Purple top tube Specimen Preparation: Allow serum tube to clot completely at room temperature. Separate serum or plasma from cells ASAP within 30 minutes of collection. Transfer 1 mL serum or plasma to a Standard Transport Tube. (Min: 0.3 mL) Storage/Transport Temperature: Unacceptable Conditions: Remarks: Stability: Reference Interval Components Available Separately Reference Interval By report (reports may vary based on instrumentation) Interpretive Data Note CPT Code 80048 Components Component Test Code Component Chart Name LOINC Aliases

Ordering information regarding a ​Rheumatoid Factor (RF) for Clinical Diagnostic Laboratories. Rheumatoid Factor (RF) Ordering Recommendation Aids in the workup of suspected rheumatoid arthritis or undifferentiated inflammatory arthritides. Consider ordering this test in conjunction with Cyclic Citrullinated Peptide (CCP) Antibody, IgG (0055256 ) to increase specificity and sensitivity. Rheumatoid Arthritis Panel (3004055 ) is the preferred test. Mnemonic Methodology Quantitative Immunoturbidimetry Performed Mon-Sat Reported Within 24 hours New York DOH Approval Status This test is New York DOH approved. Specimen Required Patient Preparation: Fasting specimen preferred. Collect: Serum separator tube or plasma separator tube. Also acceptable: Green (lithium heparin), lavender or pink. Specimen Preparation: Allow the specimen to clot completely at room temperature. Transfer 0.5 mL serum or plasma to a Standard Transport Tube. (Min: 0.5 mL) Storage/Transport Temperature: Refrigerated Unacceptable Conditions: Plasma or other body fluids. Contaminated, hemolyzed, grossly icteric, or grossly lipemic specimens. Remarks: Stability: After separation from cells: Ambient: 24 hours; Refrigerated: 8 days; Frozen: 3 months (should not be thawed more than once) Reference Interval 0-14 IU/mL Interpretive Data Note CPT Code 86431 Components Component Chart Name Rheumatoid Factor Component Test Code 0050465 LOINC 11572-5 Aliases RA RF Rheumatoid Arthritis Factor

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Ordering information regarding a Cardiolipin Antibody, IgM test for Clinical Diagnostic Laboratories. Beta-2 Glycoprotein 1 Antibody, IgA Ordering Recommendation Preferred second-line test when seronegative antiphospholipid syndrome (APS) is strongly suspected. Order incrementally or concurrently with other noncriteria antiphospholipid antibody tests. May also be useful in estimating risk of thrombosis and/or pregnancy-related morbidity in patients with SLE. Mnemonic B2GPI A Performed Mon-Sat Methodology Semi-Quantitative Enzyme-Linked Immunosorbent Assay Reported 1-2 days New York DOH Approval Status This test is New York DOH approved. Specimen Required Patient Preparation: Collect: Serum separator tube. Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Storage/Transport Temperature: Refrigerated. Unacceptable Conditions: Plasma or other body fluids. Contaminated, hemolyzed, grossly icteric, or severely lipemic specimens. Remarks: Stability: After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles) Reference Interval <=20 SAU Interpretive Data Compliance Category: FDA Note MPL = IgM phospholipid units CPT Code 86146 Components Component Chart Name B2Glycoprotein 1, IgA Antibody Component Test Code 0050324 LOINC 21108-6 Aliases Anti-B2-Glycoprotein 1 Anti-B2-Glycoprotein I Anti-B2-GP 1 Anti-B2-GP I Anti-B2-GP1 Anti-B2-GPI Anti-Beta-2-Glycoprotein I APOH Apolipoprotein H B2 IgA B2-glycoprotein 1 IgA B2GP1 IgA Beta 2GP1 Ab

Ordering information regarding a Allergen, Food, Egg Whole test for Clinical Diagnostic Laboratories. Allergen, Food, Egg Whole Ordering Recommendation Not recommended for initial thyroid disorders testing. Distinguish thyroid autoimmune disorders from nonautoimmune disease or hypothyroidism. Mnemonic EGGWHOLE Methodology Quantitative ImmunoCAP Fluorescent Enzyme Immunoassay Performed Mon-Sat Reported 1-3 days New York DOH Approval Status This test is New York DOH approved. Specimen Required Patient Preparation: Multiple patient encounters should be avoided. Collect: Serum separator tube. Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Storage/Transport Temperature: Refrigerated. Unacceptable Conditions: Grossly hemolyzed or lipemic specimens. Remarks: Stability: After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year Reference Interval Probability of IgE Mediated Clinical Reaction Reporting Range (reported in kU/L) Class Scoring No significant level detected Less than 0.10 0 0.10 - 0.34 0.35 - 0.70 Clinical relevance undetermined Low 0/1 1 0.71 - 3.50 3.51 - 17.50 Moderate High 2 3 17.51 - 50.00 50.01 - 100.00 Very high Very high 4 5 Greater than 100.00 Very high 6 Interpretive Data Allergen results of 0.10-0.34 kU/L are intended for specialist use as the clinical relevance is undetermined. Even though increasing ranges are reflective of increasing concentrations of allergen-specific IgE, these concentrations may not correlate with the degree of clinical response or skin testing results when challenged with a specific allergen. The correlation of allergy laboratory results with clinical history and in vivo reactivity to specific allergens is essential. A negative test may not rule out clinical allergy or even anaphylaxis. Compliance Category: FDA Note Components CPT Code 86003 Component Chart Name Allergen, Food, Egg Whole IgE Component Test Code 0055381 LOINC 7291-8 Aliases ImmunoCAP f245

Ordering information regarding an ACTH test for Clinical Diagnostic Laboratories. ACTH by Immunohistochemistry Ordering Recommendation Mnemonic Methodology Immunohistochemistry Performed Mon-Sat Reported 1-3 days New York DOH Approval Status This test is New York DOH approved. Specimen Required Patient Preparation: Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen (cells must be prepared into a cellblock). Protect paraffin block and/or slides from excessive heat. Transport tissue block or 5 unstained (3- to 5-micron thick sections), positively charged slides in a tissue transport kit Collect: Tissue or Cells Specimen Preparation: After drawing blood into an EDTA tube, label it with the patient's details. Keep the specimen at room temperature (15-30°C) if transporting within a few hours. For longer transport, place it in a biohazard bag within a cooler with ice packs, maintaining 2-8°C. Deliver it to the lab within 24 hours. Storage/Transport Temperature: Room temperature or refrigerated. Ship in cooled container during summer months. Unacceptable Conditions: Specimens submitted with non-representative tissue type. Depleted specimens. Remarks: Stability: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable Reference Interval Components Available Separately Reference Interval Interpretive Data Note This test is performed as a stain and return (technical) service only. CPT Code 88342 Components Component Test Code 2003428 2003429 Component Chart Name ACTH by IHC LOINC ACTH Reference Number 94736-6 Aliases ACTH ACTH Antibody Staining ACTH by IHC

Ordering information regarding a Deamidated Gliadin Peptide (DGP) Antibody, IgA test for Clinical Diagnostic Laboratories. Deamidated Gliadin Peptide (DGP) Antibody, IgA Ordering Recommendation Acceptable single screening test for celiac disease. IgA testing recommended to identify IgA deficiency. Use IgA test in individuals who are IgA competent. May be useful in diagnosing children <2 years who test negative for tTG and EMA antibodies. May aid in monitoring adherence to gluten-free diet. Celiac Disease Reflexive Cascade (2008114 ) or Tissue Transglutaminase (tTG) Antibody, IgA (0097709 ) is the preferred test for screening patients with suspected celiac disease. Mnemonic Methodology Semi-Quantitative Enzyme-Linked Immunosorbent Assay Performed Mon-Sat Reported 1-2 Days New York DOH Approval Status This test is New York DOH approved. Specimen Required Patient Preparation: Collect: Serum separator tube (SST). Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transport 0.5 mL serum. (Min: 0.3 mL) Storage/Transport Temperature: Refrigerated Unacceptable Conditions: Plasma or other body fluids. Contaminated, hemolyzed, grossly icteric, or grossly lipemic specimens. Remarks: Plasma. Hemolyzed or severely lipemic specimens. Stability: After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year Reference Interval 19 Units or less 20-30 Units Negative Weak Positive 31 Units or greater Positive Interpretive Data Note Human recombinant tTG antigen is utilized in this assay CPT Code 86258 Components Component Chart Name Deamidated Gliadin Peptide (DGP) Ab, IgA Component Test Code 0051357 LOINC 63453-5 Aliases Celiac Disease Antibodies Coeliac Disease Antib odies Deamidated Gliadin Antibodies (DGP) Deaminated Gliadin Peptide Antibodies DGP DGP Ab DGP IgA

Ordering information regarding a PTINR test for Clinical Diagnostic Laboratories. Prothrombin Time/International Normalized Ratio (PTINR) Ordering Recommendation Initial test for evaluating bleeding disorders and monitoring oral anticoagulation therapy (warfarin/Coumadin). Methodology Electromagnetic Mechanical Clot Detection Performed Mon-Sat Reported 1-2 days New York DOH Approval Status This test is New York DOH approved. Specimen Required Patient Preparation: Collect: Lt. blue (sodium citrate) Specimen Preparation: Transfer 1 mL platelet-poor plasma to a Standard Transport Tube. (Min: 0.5 mL) Storage/Transport Temperature: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered. Unacceptable Conditions: Serum. EDTA plasma, clotted or hemolyzed specimens. Remarks: Stability: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: 2 weeks Reference Interval Components Available Separately Reference Interval International Normalized Ratio Coumadin Therapy: INR: 2.0-3.0 conventional anticoagulation INR: 2.5-3.5 intensive anticoagulation Prothrombin Time 12.0-15.5 seconds Interpretive Data Note Reference intervals established using 3.2 percent sodium citrate. CPT Code 85610 Components Component Test Code Component Chart Name LOINC 0030220 Prothrombin Time 5902-2 0030223 International Normalized Ratio 6301-6 Aliases INR Pro Time Protime Protime, PT/INR PT/International Normalized Ratio

Ordering information regarding a Tissue Transglutaminase (tTG) IgG test for Clinical Diagnostic Laboratories. Tissue Transglutaminase (tTG) IgG Ordering Recommendation Recommended single screening test for IgA-deficient individuals with suspected celiac disease. (IgA testing recommended to identify IgA deficiency.) May aid in monitoring adherence to gluten-free diet in celiac disease confirmed patients. Mnemonic Methodology Semi-Quantitative Enzyme-Linked Immunosorbent Assay Performed Mon-Sat Reported 1-2 Days New York DOH Approval Status This test is New York DOH approved. Specimen Required Patient Preparation: Collect: Serum separator tube (SST). Specimen Preparation: Remove serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to a Standard Transport Tube. (Min: 0.5 mL) Storage/Transport Temperature: Refrigerated Unacceptable Conditions: Refrigerated. Remarks: Plasma. Hemolyzed or severely lipemic specimens. Stability: After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year Reference Interval 5 U/mL or less 6-9 U/mL Negative Weak Positive 10 U/mL or greater Positive Interpretive Data The tTG IgG assay may aid in the diagnosis of gluten-sensitivity enteropathy (i.e., celiac disease, dermatitis herpetiformis) in tTG IgA negative patients with confirmed IgA deficiency. A negative tTG IgG test alone does not rule out gluten-sensitive enteropathy. Note Human recombinant tTG antigen is utilized in this assay CPT Code 86364 Components Component Chart Name Tissue Transglutaminase (tTG) Ab, IgA Component Test Code 0056009 LOINC 56537-4 Aliases Tissue Transglutaminase Transglu taminase tTG tTG IgG

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