Double-Stranded DNA (DsDNA) Antibody

Ordering Recommendation

Secondary screening for systemic lupus erythematosus (SLE) based on ANA results.

Mnemonic

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody

Performed

Mon-Sat

Reported

1-3 Days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation:

Collect:

Serum separator tube (SST).

Specimen Preparation:

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to a Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature:

Refrigerated

Unacceptable Conditions:

Plasma. Contaminated, hemolyzed, or severely lipemic specimens.

Remarks:

Stability:

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 month (avoid repeated freeze/thaw cycles)

Reference Interval

24 IUs or less

Double-Stranded DNA (dsDNA) Ab IgG ELISA

Interpretive Data

Positivity for anti-double stranded DNA (anti-dsDNA) IgG antibody is a diagnostic criterion of systemic lupus erythematosus (SLE). Specimens are initially screened by enzyme-linked immunosorbent assay (ELISA). If ordered as reflex (0050215), positive ELISA results (>24 IU) will be reflexed to a highly specific IFA titer (Crithidia luciliae indirect fluorescent test [CLIFT]) for confirmation. Some patients with early or inactive SLE may be positive for anti-dsDNA IgG by ELISA but negative by CLIFT. If the patient is negative by CLIFT but positive by ELISA and clinical suspicion remains, consider antinuclear antibody (ANA) testing by IFA.

Note

If dsDNA IgG antibody is detected, the result will be confirmed by Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae). Additional charges apply.

CPT Code

86225; if reflexed, add 86256

Components

Component Chart Name

Component Test Code

LOINC

Double-Stranded DNA (dsDNA) Ab IgG ELISA

0050215

58465-6

Aliases

anti-DNA

Anti-dsDNA Antibody

Anti-Native DNA

Antibody to ds-DNA

Crithidia luciliae

DNA ds Ab

Double stranded DNA antibody

ds DNA Ab

ds-DNA

ds-DNA IgG

dsDNA reflex

dsDNA reflex panel

Native double-stranded DNA antibody